Robustness analysis

Robustness analysis can be used to study the effect of changing a reaction flux on the other reaction flux, especially on the objective of interest (e.g. growth rate). Therefore, you can select the reactions of interest in the model for robustness analysis.

To see how sensitive of the objective reaction (Biomass) is to the glucose uptake rate in the range of -20 to 0 mmol/gDW/h.

Robustness analysis for growth rate maximization while changing glucose uptake rate (uptaking 0-20 mmol/gDW/h) with oxygen uptake fixed at 17 mmol/gDW/h (set LB to -17)

To see how sensitive of the objective reaction (Biomass) is to the oxygen uptake rate in the range of -25 to 0 mmol/gDW/h.

Robustness analysis for growth rate maximization while changing oxygen uptake rate (uptaking 0-25 mmol/gDW/h) with glucose uptake fixed at 10 mmol/gDW/h (set LB to -10).

To change two reactions simultaneously. GEMSiRV can plot the results as a phenotypic phase plane.

The phenotypic phase plane for growth rate maximization while changing glucose and oxygen uptake rates in the range of -20 to 0 mmol/ gDW/h.