To constrain a reaction in a zero flux can simulate the reaction deletion. Likewise, to constrain the reaction corresponding to a deleted gene can simulate the gene deletion. GEMSiRV performs essentiality analysis for gene and reaction separately and determines the rate ratio (objective flux of deleted model to that of wild-type model) for every single-knockout condition.
You can select a metabolic model for essentiality analysis, the computational essential genes or reactions can be identified.
Results of gene essentiality analysis:
Results of reaction essentiality analysis: