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Paired reads are available at Illumina Miseq (Mate1, Mate2)
Read length: 151bp
Read amount: 5,729,470 X2
Insert size ~ 300bp
We combined MiSeq_Ecoli_MG1655_110721_PF_R1.fastq and MiSeq_Ecoli_MG1655_110721_PF_R2.fastq to MiSeq_PE.fastq.
java convertFastqToFastaAndQual MiSeq_PE.fastq MiSeq_PE.fna MiSeq_PE.qual convert-fasta-to-v2.pl -mean 297 -stddev 35 -m Mate_info -l Illumia_Ecoli -s MiSeq_PE.fna -q MiSeq_PE.qual > MiSeq_PE.frg
The data in frg format were downloaded from Miseq100X.frg
We have trimmed the sequence reads to be of error probability less than 0.05. The paired-end reads were discarded if one read is shorter than 150bp.
We therefore obtained 1,839,935 paired-end reads (~118X, tMiSeq_PE.frg) with high quality for further analysis.
17 SMRT Cells for E. coli MG1655 were downloaded (details in Data and Read Depths) and filtered with smrtpipe.
Abyss, Edena, SPAdes, SOAPdenovo2, Velvet, CISA (Note at: 20130718_MiSeq_with_verious_Assemblers) MaSuRCA (note at: MaSuRCA for MiSeq Data)
runCA (Details can be seen in Read Depths, pacBioToCA and runCA), MIRA3 (PacBio 17 SMRT CELL)
AHA, PBJelly, Cerulean, Patch