QUAST report
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Contigs are ordered from largest (contig #1) to smallest. Contigs are broken into nonoverlapping 100 bp windows. Plot shows numbers of windows for each GC percentage. |
{"date":"17 March 2014, Monday, 13:13:32","report":[["Statistics without reference",[{"values":[1,1,1,5,2,2,1,4,1,2],"quality":"Less is better","isMain":true,"metricName":"# contigs"},{"values":[1,1,1,5,2,2,1,4,1,2],"quality":"Less is better","isMain":false,"metricName":"# contigs (>= 0 bp)"},{"values":[1,1,1,5,2,2,1,4,1,2],"quality":"Less is better","isMain":false,"metricName":"# contigs (>= 1000 bp)"},{"values":[4651604,4647117,4648057,3447068,3749516,2770859,4649699,1679082,4649323,4189785],"quality":"More is better","isMain":true,"metricName":"Largest contig"},{"values":[4651604,4647117,4648057,4661453,4645941,4657272,4649699,4655949,4649323,4652482],"quality":"More is better","isMain":true,"metricName":"Total length"},{"values":[4651604,4647117,4648057,4661453,4645941,4657272,4649699,4655949,4649323,4652482],"quality":"More is better","isMain":false,"metricName":"Total length (>= 0 bp)"},{"values":[4651604,4647117,4648057,4661453,4645941,4657272,4649699,4655949,4649323,4652482],"quality":"More is better","isMain":false,"metricName":"Total length (>= 1000 bp)"},{"values":[4651604,4647117,4648057,3447068,3749516,2770859,4649699,1159845,4649323,4189785],"quality":"More is better","isMain":true,"metricName":"N50"},{"values":[4651604,4647117,4648057,656513,3749516,1886413,4649699,983561,4649323,4189785],"quality":"More is better","isMain":false,"metricName":"N75"},{"values":[1,1,1,1,1,1,1,2,1,1],"quality":"Less is better","isMain":false,"metricName":"L50"},{"values":[1,1,1,2,1,2,1,3,1,1],"quality":"Less is better","isMain":false,"metricName":"L75"},{"values":["50.79","50.79","50.79","50.80","50.78","50.77","50.78","50.79","50.78","50.79"],"quality":"Equal","isMain":false,"metricName":"GC (%)"}]],["Misassemblies",[{"values":[9,7,8,7,7,10,10,6,10,8],"quality":"Less is better","isMain":true,"metricName":"# misassemblies"},{"values":[8,7,8,7,7,10,10,6,10,8],"quality":"Less is better","isMain":false,"metricName":" # relocations"},{"values":[0,0,0,0,0,0,0,0,0,0],"quality":"Less is better","isMain":false,"metricName":" # translocations"},{"values":[1,0,0,0,0,0,0,0,0,0],"quality":"Less is better","isMain":false,"metricName":" # inversions"},{"values":[1,1,1,1,2,2,1,2,1,1],"quality":"Less is better","isMain":false,"metricName":"# misassembled contigs"},{"values":[4651604,4647117,4648057,3447068,4645941,4657272,4649699,2143406,4649323,4189785],"quality":"Less is better","isMain":true,"metricName":"Misassembled contigs length"},{"values":[3,4,3,4,2,1,3,4,4,4],"quality":"Less is better","isMain":false,"metricName":"# local misassemblies"}]],["Unaligned",[{"values":[0,0,0,0,0,0,0,0,0,0],"quality":"Less is better","isMain":false,"metricName":"# fully unaligned contigs"},{"values":[0,0,0,0,0,0,0,0,0,0],"quality":"Less is better","isMain":false,"metricName":"Fully unaligned length"},{"values":[0,0,0,0,0,0,0,0,0,0],"quality":"Less is better","isMain":false,"metricName":"# partially unaligned contigs"},{"values":[0,0,0,0,0,0,0,0,0,0],"quality":"Less is better","isMain":false,"metricName":" # 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short indels (<= 5 bp)"},{"values":[0,0,0,0,0,0,0,0,0,0],"quality":"Less is better","isMain":false,"metricName":" # long indels (> 5 bp)"},{"values":[0,1,0,0,0,0,0,0,1,0],"quality":"Less is better","isMain":false,"metricName":"# N's"},{"values":["0.00","0.02","0.00","0.00","0.00","0.00","0.00","0.00","0.02","0.00"],"quality":"Less is better","isMain":true,"metricName":"# N's per 100 kbp"}]],["Genome statistics",[{"values":["100.000","100.000","100.000","99.949","99.956","100.000","100.000","99.959","100.000","99.993"],"quality":"More is better","isMain":true,"metricName":"Genome fraction (%)"},{"values":["1.003","1.002","1.002","1.005","1.002","1.005","1.002","1.004","1.002","1.003"],"quality":"Less is better","isMain":true,"metricName":"Duplication ratio"},{"values":["4494 + 3 part","4494 + 3 part","4494 + 3 part","4490 + 5 part","4491 + 2 part","4495 + 2 part","4494 + 3 part","4489 + 6 part","4494 + 3 part","4493 + 4 part"],"quality":"More is better","isMain":true,"metricName":"# genes"},{"values":[2231751,1907985,1569022,1757508,2194509,1570181,2995552,1677251,2956338,1428683],"quality":"More is better","isMain":false,"metricName":"Largest alignment"},{"values":[4651604,4647117,4648057,3447068,3749516,2770859,4649699,1159845,4649323,4189785],"quality":"More is better","isMain":false,"metricName":"NG50"},{"values":[4651604,4647117,4648057,656513,3749516,1886413,4649699,983561,4649323,4189785],"quality":"More is better","isMain":false,"metricName":"NG75"},{"values":[1207212,1428636,1432247,983650,1552642,873232,2995552,1062313,2956338,1207192],"quality":"More is better","isMain":false,"metricName":"NA50"},{"values":[770880,1219471,1207132,616842,1552642,574574,1207206,982694,1207204,1114225],"quality":"More is better","isMain":false,"metricName":"NA75"},{"values":[1207212,1428636,1432247,983650,1552642,873232,2995552,1062313,2956338,1207192],"quality":"More is better","isMain":true,"metricName":"NGA50"},{"values":[770880,1219471,1207132,616842,1552642,574574,1207206,982694,1207204,1114225],"quality":"More is better","isMain":false,"metricName":"NGA75"},{"values":[1,1,1,1,1,1,1,2,1,1],"quality":"Less is better","isMain":false,"metricName":"LG50"},{"values":[1,1,1,2,1,2,1,3,1,1],"quality":"Less is better","isMain":false,"metricName":"LG75"},{"values":[2,2,2,2,2,2,1,2,1,2],"quality":"Less is better","isMain":false,"metricName":"LA50"},{"values":[3,3,3,4,2,4,2,3,2,3],"quality":"Less is better","isMain":false,"metricName":"LA75"},{"values":[2,2,2,2,2,2,1,2,1,2],"quality":"Less is better","isMain":false,"metricName":"LGA50"},{"values":[3,3,3,4,2,4,2,3,2,3],"quality":"Less is better","isMain":false,"metricName":"LGA75"}]],["Predicted genes",[]],["Reference statistics",[{"values":[4639675,4639675,4639675,4639675,4639675,4639675,4639675,4639675,4639675,4639675],"quality":"Equal","isMain":false,"metricName":"Reference length"},{"values":["50.79","50.79","50.79","50.79","50.79","50.79","50.79","50.79","50.79","50.79"],"quality":"Equal","isMain":false,"metricName":"Reference GC (%)"},{"values":[4497,4497,4497,4497,4497,4497,4497,4497,4497,4497],"quality":"Equal","isMain":false,"metricName":"Reference genes"},{"values":[0,0,0,0,0,0,0,0,0,0],"quality":"Equal","isMain":false,"metricName":"Reference operons"}]]],"minContig":500,"assembliesNames":["All_Data","4_SMRT_cells:1st_Set","4_SMRT_cells:2nd_Set","4_SMRT_cells:3rd_Set","6_SMRT_cells:1st_Set","6_SMRT_cells:2nd_Set","6_SMRT_cells:3rd_Set","8_SMRT_cells:1st_Set","8_SMRT_cells:2nd_Set","8_SMRT_cells:3rd_Set"]}
{{ qualities }}
{{ mainMetrics }}
{"lists_of_lengths":[[4651604],[4647117],[4648057],[3447068,656513,374632,138151,45089],[3749516,896425],[2770859,1886413],[4649699],[1679082,1159845,983561,833461],[4649323],[4189785,462697]],"filenames":["All_Data","4_SMRT_cells:1st_Set","4_SMRT_cells:2nd_Set","4_SMRT_cells:3rd_Set","6_SMRT_cells:1st_Set","6_SMRT_cells:2nd_Set","6_SMRT_cells:3rd_Set","8_SMRT_cells:1st_Set","8_SMRT_cells:2nd_Set","8_SMRT_cells:3rd_Set"]}
{"lists_of_lengths":[[2231751,1207212,770880,603611,89874,1819,1818,1205,769,769],[1907985,1428636,1219471,89868,1818,1205,769,769],[1569022,1432247,1207132,603564,89869,1818,1205,769,769],[1757508,983650,656513,616842,374632,138151,89870,45089,1817,1208,769,768],[2194509,1552642,572337,232187,89895,2034,1200,769,768],[1570181,873232,627539,574574,572326,236035,202527,1808,1200,771,768,97],[2995552,1207206,603608,89895,4652,1839,1819,1205,780,769,769],[1677251,1062313,982694,833461,89673,5670,1195,769,768,99],[2956338,1207204,643264,89878,3395,1839,1819,1205,801,769,769],[1428683,1207192,1114225,603603,462657,89872,1818,1205,769,769]],"filenames":["All_Data","4_SMRT_cells:1st_Set","4_SMRT_cells:2nd_Set","4_SMRT_cells:3rd_Set","6_SMRT_cells:1st_Set","6_SMRT_cells:2nd_Set","6_SMRT_cells:3rd_Set","8_SMRT_cells:1st_Set","8_SMRT_cells:2nd_Set","8_SMRT_cells:3rd_Set"]}
{"assemblies_lengths":[4651604,4647117,4648057,4661453,4645941,4657272,4649699,4655949,4649323,4652482],"filenames":["All_Data","4_SMRT_cells:1st_Set","4_SMRT_cells:2nd_Set","4_SMRT_cells:3rd_Set","6_SMRT_cells:1st_Set","6_SMRT_cells:2nd_Set","6_SMRT_cells:3rd_Set","8_SMRT_cells:1st_Set","8_SMRT_cells:2nd_Set","8_SMRT_cells:3rd_Set"]}
{"reflen":4639675}
{"ref_genes_number":4497,"genes_in_contigs":{"8_SMRT_cells:2nd_Set":[4494],"4_SMRT_cells:1st_Set":[4494],"8_SMRT_cells:3rd_Set":[4053,440],"4_SMRT_cells:3rd_Set":[3374,603,357,118,38],"4_SMRT_cells:2nd_Set":[4494],"6_SMRT_cells:2nd_Set":[2620,1875],"8_SMRT_cells:1st_Set":[1629,1131,968,761],"6_SMRT_cells:3rd_Set":[4494],"All_Data":[4494],"6_SMRT_cells:1st_Set":[3582,909]},"filenames":["All_Data","4_SMRT_cells:1st_Set","4_SMRT_cells:2nd_Set","4_SMRT_cells:3rd_Set","6_SMRT_cells:1st_Set","6_SMRT_cells:2nd_Set","6_SMRT_cells:3rd_Set","8_SMRT_cells:1st_Set","8_SMRT_cells:2nd_Set","8_SMRT_cells:3rd_Set"]}
{{ operonsInContigs }}
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{
"# contigs" : "is the total number of contigs in the assembly.",
"Largest contig" : "is the length of the longest contig in the assembly.",
"Total length" : "is the total number of bases in the assembly.",
"Reference length" : "is the total number of bases in the reference.",
"N50" : "is the contig length such that using longer or equal length contigs produces half (50%) of the bases of the assembly. Usually there is no value that produces exactly 50%, so the technical definition is the maximum length x such that using contigs of length at least x accounts for at least 50% of the total assembly length.",
"NG50" : "is the contig length such that using longer or equal length contigs produces half (50%) of the bases of the reference genome. This metric is computed only if a reference genome is provided.",
"N75" : "is the contig length such that using longer or equal length contigs produces 75% of the bases of the assembly. Usually there is no value that produces exactly 75%, so the technical definition is the maximum length x such that using contigs of length at least x accounts for at least 75% of the total assembly length.",
"NG75" : "is the contig length such that using longer or equal length contigs produces 75% of the bases of the reference genome. This metric is computed only if a reference genome is provided.",
"L50" : "is the minimum number of contigs that produce half (50%) of the bases of the assembly. In other words, it's the number of contigs of length at least N50.",
"LG50" : "is the minimum number of contigs that produce half (50%) of the bases of the reference genome. In other words, it's the number of contigs of length at least NG50. This metric is computed only if a reference genome is provided.",
"L75" : "is the minimum number of contigs that produce 75% of the bases of the assembly. In other words, it's the number of contigs of length at least N75.",
"LG75" : "is the minimum number of contigs that produce 75% of the bases of the reference genome. In other words, it's the number of contigs of length at least NG75. This metric is computed only if a reference genome is provided.",
"NA50" : "is N50 where the lengths of aligned blocks are counted instead of contig lengths. I.e., if a contig has a misassembly with respect to the reference, the contig is broken into smaller pieces. This metric is computed only if a reference genome is provided.",
"NGA50" : "is NG50 where the lengths of aligned blocks are counted instead of contig lengths. I.e., if a contig has a misassembly with respect to the reference, the contig is broken into smaller pieces. This metric is computed only if a reference genome is provided.",
"NA75" : "is N75 where the lengths of aligned blocks are counted instead of contig lengths. I.e., if a contig has a misassembly with respect to the reference, the contig is broken into smaller pieces. This metric is computed only if a reference genome is provided.",
"NGA75" : "is NG75 where the lengths of aligned blocks are counted instead of contig lengths. I.e., if a contig has a misassembly with respect to the reference, the contig is broken into smaller pieces. This metric is computed only if a reference genome is provided.",
"LA50" : "is L50 where aligned blocks are counted instead of contigs. I.e., if a contig has a misassembly with respect to the reference, the contig is broken into smaller pieces.",
"LGA50" : "is LG50 where aligned blocks are counted instead of contigs. I.e., if a contig has a misassembly with respect to the reference, the contig is broken into smaller pieces.",
"LA75" : "is L75 where aligned blocks are counted instead of contigs. I.e., if a contig has a misassembly with respect to the reference, the contig is broken into smaller pieces.",
"LGA75" : "is LG75 where aligned blocks are counted instead of contigs. I.e., if a contig has a misassembly with respect to the reference, the contig is broken into smaller pieces.",
"Average %IDY" : "is the average of alignment identity percent (Nucmer measure of alignment accuracy) among all contigs.",
"# misassemblies" : "is the number of positions in the assembled contigs where the left flanking sequence aligns over 1 kbp away from the right flanking sequence on the reference (relocation) or they overlap on more than 1 kbp (relocation) or flanking sequences align on different strands (inversion) or different chromosomes (translocation).",
"# misassembled contigs" : "is the number of contigs that contain misassembly events.",
"Misassembled contigs length" : "is the number of total bases contained in all contigs that have one or more misassemblies.",
"# relocations" : "is the number of relocation events among all misassembly events. Relocation is a misassembly where the left flanking sequence aligns over 1 kbp away from the right flanking sequence on the reference, or they overlap by more than 1 kbp and both flanking sequences align on the same chromosome.",
"# translocations" : "is the number of translocation events among all misassembly events. Translocation is a misassembly where the flanking sequences align on different chromosomes.",
"# inversions" : "is the number of inversion events among all misassembly events. Inversion is a misassembly where it is not a relocation and the flanking sequences align on opposite strands of the same chromosome.",
"# local misassemblies" : "is the number of local misassemblies. We define a local misassembly breakpoint as a breakpoint that satisfies these conditions:
- Two or more distinct alignments cover the breakpoint.
- The gap between left and right flanking sequences is less than 1 kbp.
- The left and right flanking sequences both are on the same strand of the same chromosome of the reference genome.