(→Sequence assembly)
|
(→Sequence assembly)
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Line 25: | |||
The above assemblers together with the parameter setting have been selected for ''de novo'' assembling of ''E. coli''. | The above assemblers together with the parameter setting have been selected for ''de novo'' assembling of ''E. coli''. | ||
After assembly, we discarded contigs with less than 100bp and evaluated the accuracy of the assemblies based on the Mauve Assembly Matrices (the results are shown below). | After assembly, we discarded contigs with less than 100bp and evaluated the accuracy of the assemblies based on the Mauve Assembly Matrices (the results are shown below). | ||
+ | In this set of data, we have different sequence assemblies, each was generated by the different assembler. | ||
+ | Because even the same assemble performs differently over varying parameter settings such as kmer, we have tried different parameter settings for Abyss and SOAPdenovo in the following sets. | ||
* '''Set2''' (Different parameters for Abyss - the assembler provides the lowest number of contigs in Set1) | * '''Set2''' (Different parameters for Abyss - the assembler provides the lowest number of contigs in Set1) |