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- | [^] Please note that the ContigN50 calculated by Mauve Assembly Metrics is incorrect (off-by-one error). We have followed the definition of N50 (''A contig N50 is calculated by first ordering every contig by length from longest to shortest. Next, starting from the longest contig, the lengths of each contig are summed, until this running sum equals one-half of the total length of all contigs in the assembly. The contig N50 of the assembly is the length of the shortest contig in this list.'') [[http://www.ncbi.nlm.nih.gov/pubmed/22510764 ref]] to calculate N50s. The N50 contig size calculated by GAGE using the total reference genome length rather than the sum total of contig lengths. GAGE's cor.N50 values were computed after correcting contigs by breaking them at each error. | + | [^] Please note that the ContigN50 calculated by Mauve Assembly Metrics is incorrect (off-by-one error). We have followed the definition of N50 (''A contig N50 is calculated by first ordering every contig by length from longest to shortest. Next, starting from the longest contig, the lengths of each contig are summed, until this running sum equals one-half of the total length of all contigs in the assembly. The contig N50 of the assembly is the length of the shortest contig in this list.'') [http://www.ncbi.nlm.nih.gov/pubmed/22510764 ref] to calculate N50s. The N50 contig size calculated by GAGE using the total reference genome length rather than the sum total of contig lengths. GAGE's cor.N50 values were computed after correcting contigs by breaking them at each error. |
[<sup>#</sup>] Please note that GAA and minimus2 were designed to merge two assemblies at a time, we thus performed all [http://sb.nhri.org.tw/CISA/en/E_coli_36_all2 runs] and took the average scores. | [<sup>#</sup>] Please note that GAA and minimus2 were designed to merge two assemblies at a time, we thus performed all [http://sb.nhri.org.tw/CISA/en/E_coli_36_all2 runs] and took the average scores. |