(→100X)
|
(→S. pneumoniae)
|
Line 122: | |||
=S. pneumoniae= | =S. pneumoniae= | ||
Streptococcus pneumoniae TIGR4. The S. pneumoniae TIGR4 consists of a circular chromosome of 2,160,842 bp in length. | Streptococcus pneumoniae TIGR4. The S. pneumoniae TIGR4 consists of a circular chromosome of 2,160,842 bp in length. | ||
+ | ==Website data== | ||
+ | The Illumina and pacbio data were downloaded from [ftp://ftp.broadinstitute.org/pub/papers/assembly/Ribeiro2012/data/rhody_data.tar.gz ALLPATHS-LG website] <br> | ||
+ | ===Fragment library=== | ||
+ | Reads length : 101bp <br> | ||
+ | Reads amount : 4354215 X2 <br> | ||
+ | Insert size : 180bp <br> | ||
+ | Coverage : 170.16X | ||
+ | ===Jumping library=== | ||
+ | Reads length : 101bp <br> | ||
+ | Reads amount : 1974031 X2 <br> | ||
+ | Insert size : 3000bp <br> | ||
+ | ===PacBio reads=== | ||
+ | Reads average length : 1031.19bp <br> | ||
+ | Reads amount : 1994107 <br> | ||
+ | Coverage : 446.44X <br> | ||
+ | ==Raw data== | ||
+ | The raw data of website data from Sequence Read Archive (SRA) | ||
+ | ===Fragment library=== | ||
+ | Accession : [http://www.ncbi.nlm.nih.gov/sra/SRX000946 SRX000946] <br> | ||
+ | Reads length : 101bp <br> | ||
+ | Reads amount : 11339101 X2 <br> | ||
+ | Insert size : 180bp <br> | ||
+ | Coverage : 433.12X <br> | ||
+ | ===Jumping library=== | ||
+ | Accession : [http://www.ncbi.nlm.nih.gov/sra/SRX111018 SRX111018] <br> | ||
+ | ===PacBio reads=== | ||
+ | Accession : [http://www.ncbi.nlm.nih.gov/sra/SRX109847 SRX109847](SRR386702), [http://www.ncbi.nlm.nih.gov/sra/SRX109812 SRX109812],[http://www.ncbi.nlm.nih.gov/sra/SRX109830 SRX109830],[http://www.ncbi.nlm.nih.gov/sra/SRX109818 SRX109818](SRR386746),[http://www.ncbi.nlm.nih.gov/sra/SRX111329 SRX111329] | ||
+ | ==Self-fraction data== | ||
+ | We randomly selected the same fraction as website data from fragment library of raw data by prepare.sh. | ||
+ | PrepareAllPathsInputs.pl\ | ||
+ | DATA_DIR=$PWD/test.genome/data\ | ||
+ | PLOIDY=1\ | ||
+ | FRAG_FRAC=0.384\ | ||
+ | IN_GROUPS_CSV=in_groups.csv\ | ||
+ | IN_LIBS_CSV=in_libs.csv\ | ||
+ | OVERWRITE=True\ | ||
+ | | tee prepare.out | ||
+ | ==100X== | ||
+ | We randomly selected 100X coverage data from fragment library of raw data by prepare.sh. | ||
+ | Fraction = 100/443.12 = 0.226 <br> | ||
+ | PrepareAllPathsInputs.pl\ | ||
+ | DATA_DIR=$PWD/test.genome/data\ | ||
+ | PLOIDY=1\ | ||
+ | FRAG_FRAC=0.226\ | ||
+ | IN_GROUPS_CSV=in_groups.csv\ | ||
+ | IN_LIBS_CSV=in_libs.csv\ | ||
+ | OVERWRITE=True\ | ||
+ | | tee prepare.out |