Version Differences for Dataset1
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(→Short reads)
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(→Short reads)
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| Line 3: | |||
| The paired-end illumina read data of ''E. coli'' were downloaded from Illumina ([http://www.illumina.com/truseq/tru_resources/datasets.ilmn |Illumina]) with a median insert size of 214 bp. More than 28.4 M reads. | The paired-end illumina read data of ''E. coli'' were downloaded from Illumina ([http://www.illumina.com/truseq/tru_resources/datasets.ilmn |Illumina]) with a median insert size of 214 bp. More than 28.4 M reads. | ||
| + | We converted the required format using Picard tools ([http://picard.sourceforge.net/command-line-overview.shtml ref]). | ||
| java -jar SamToFastq.jar INPUT=Ecoli_MG1655_s_6_1_bfast.bam FASTQ=Ecoli_MG1655_s1.fastq | java -jar SamToFastq.jar INPUT=Ecoli_MG1655_s_6_1_bfast.bam FASTQ=Ecoli_MG1655_s1.fastq | ||
| java -jar SamToFastq.jar INPUT=Ecoli_MG1655_s_6_2_bfast.bam FASTQ=Ecoli_MG1655_s2.fastq | java -jar SamToFastq.jar INPUT=Ecoli_MG1655_s_6_2_bfast.bam FASTQ=Ecoli_MG1655_s2.fastq | ||
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| - | We have combined MiSeq_Ecoli_MG1655_110721_PF_R1.fastq and MiSeq_Ecoli_MG1655_110721_PF_R2.fastq to MiSeq_PE.fastq. | + | We combined MiSeq_Ecoli_MG1655_110721_PF_R1.fastq and MiSeq_Ecoli_MG1655_110721_PF_R2.fastq to MiSeq_PE.fastq. |
| java convertFastqToFastaAndQual MiSeq_PE.fastq MiSeq_PE.fna MiSeq_PE.qual | java convertFastqToFastaAndQual MiSeq_PE.fastq MiSeq_PE.fna MiSeq_PE.qual | ||