(→Long reads)
|
(→PacBio corrected reads (PBcR))
|
Line 21: | |||
= PacBio corrected reads (PBcR) = | = PacBio corrected reads (PBcR) = | ||
+ | To correct the PacBio CLR with raw short reads: | ||
pacBioToCA -length 1000 -partitions 200 -l PacBio_Illumia -s pacbio.spec -fastq PacBio_10kb_CLR.fastq Ecoli_MG1655_PE.frg 1>PacBio_Illumia_Ecoli.out 2>error.out | pacBioToCA -length 1000 -partitions 200 -l PacBio_Illumia -s pacbio.spec -fastq PacBio_10kb_CLR.fastq Ecoli_MG1655_PE.frg 1>PacBio_Illumia_Ecoli.out 2>error.out | ||
+ | To correct the PacBio CLR with 100X high-quality reads (p<0.05, length of paired-end read>=100bp) | ||
+ | pacBioToCA -length 500 -partitions 200 -l PacBio_Illumia -s pacbio.spec -fastq PacBio_10kb_CLR.fastq 100X_Ecoli_PE.frg | ||
= Assemblies with hybrid methods = | = Assemblies with hybrid methods = | ||