(→Hierarchical genome-assembly process)
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(→Hierarchical genome-assembly process)
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Line 7: | |||
- | Step 1 : Prepare for HGAP Protocol | + | '''Prepare for HGAP Protocol''' |
1. Build input XML file (detail step please refer to the [https://github.com/PacificBiosciences/SMRT-Analysis/wiki/SMRT-Pipe-Reference-Guide-v2.0#PipeInputs tutorial]) <br> | 1. Build input XML file (detail step please refer to the [https://github.com/PacificBiosciences/SMRT-Analysis/wiki/SMRT-Pipe-Reference-Guide-v2.0#PipeInputs tutorial]) <br> | ||
- | 2. Build HGAP parameters XML file : [[Media:HGAP.xml|HGAP.xml]]. we used default parameter setting mostly, | + | 2. Build HGAP parameters XML file : [[Media:HGAP.xml|HGAP.xml]]. we used default parameters setting mostly, and setting minSubReadLength = 50, readScore = 0.75, minLength = 50 here ) |
- | [Note] : Please check the "minSubReadLength", "readScore", "minLength", Genome size (Setting minSubReadLength = 50, readScore = 0.75, minLength = 50 here ) | ||
3. smrtpipe.py --params=HGAP.xml xml:input.xml (~10hr for one data set) | 3. smrtpipe.py --params=HGAP.xml xml:input.xml (~10hr for one data set) | ||