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pacbioToCA estimates genome_size, It's will get exact genome_size without genome_size parameter when there are only one or two SMRT cell.
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When amount of SMRT cell is bigger than 2, pacbioToCA with genome_size parameter will halp it get exact genome_size.
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pacBioToCA -l viaMiseq -s pacbio.spec -t 10 -partitions 200 fastqFile=m120228_192221.fastq genomeSize=4650000 ../../tMiSeq_PE.frg |
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pacBioToCA -l viaMiseq -s pacbio.spec -t 10 -partitions 200 fastqFile=m120228_192221.fastq genomeSize=4650000 ../../tMiSeq_PE.frg |
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depth: 15.97X => 看起來在只有一組的情況下,沒有差很多 |
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depth: 15.97X => 看起來在只有一組的情況下,沒有差很多 |
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pacBioToCA -l viaMiseq -s pacbio.spec -t 10 -partitions 200 fastqFile=m120228_210845.fastq genomeSize=4650000 ../../tMiSeq_PE.frg
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m120228_210845_42129_c000304152550000001500000112311370_s1_p0.fastq
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(without genomeSize)PacBio_Illumia.fasta
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depth: 18.46X => 看起來在只有一組的情況下,沒有差很多
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pacBioToCA -l viaMiseq -s pacbio.spec -t 10 -partitions 200 fastqFile=Filtered_two.fastq genomeSize=4650000 ../tMiSeq_PE.frg |
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pacBioToCA -l viaMiseq -s pacbio.spec -t 10 -partitions 200 fastqFile=Filtered_two.fastq genomeSize=4650000 ../tMiSeq_PE.frg |
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depth: 29.98X => 看起來在只有二組的情況下,沒有差很多 |
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depth: 29.98X => 看起來在只有二組的情況下,沒有差很多 |
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pacBioToCA -l viaMiseq -s pacbio.spec -t 10 -partitions 200 fastqFile=Filtered_three.fastq genomeSize=4650000 ../tMiSeq_PE.frg |
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pacBioToCA -l viaMiseq -s pacbio.spec -t 10 -partitions 200 fastqFile=Filtered_three.fastq genomeSize=4650000 ../tMiSeq_PE.frg |
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depth: 42.67X => 看起來要有三組以上的的情況下,genomeSize才有效果。 |
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depth: 42.67X => 看起來要有三組以上的的情況下,genomeSize才有效果。 |
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pacBioToCA -l viaMiseq -s pacbio.spec -t 10 -partitions 200 fastqFile=Filtered_four.fastq genomeSize=4650000 ../tMiSeq_PE.frg |
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pacBioToCA -l viaMiseq -s pacbio.spec -t 10 -partitions 200 fastqFile=Filtered_four.fastq genomeSize=4650000 ../tMiSeq_PE.frg |