(→123)
|
(→Performance)
|
Line 642: | |||
We used all SMRT cells and randomly selected four SMRT cells three times for each, and evaluated the assemblies by QUAST against the reference genome ([ftp://ftp.ncbi.nih.gov/genomes/Bacteria/Pedobacter_heparinus_DSM_2366_uid59111/ NC_013061]) and [[Media:gene_phep.ncbi | Ph_gene_list]]. ([http://sb.nhri.org.tw/comps/quast/Non-hybrid/PBcR_pipeline/D8/report.html more detail]) | We used all SMRT cells and randomly selected four SMRT cells three times for each, and evaluated the assemblies by QUAST against the reference genome ([ftp://ftp.ncbi.nih.gov/genomes/Bacteria/Pedobacter_heparinus_DSM_2366_uid59111/ NC_013061]) and [[Media:gene_phep.ncbi | Ph_gene_list]]. ([http://sb.nhri.org.tw/comps/quast/Non-hybrid/PBcR_pipeline/D8/report.html more detail]) | ||
==Performance== | ==Performance== | ||
+ | {| {{table}} border="1" | ||
+ | | align="center" style="background:#f0f0f0;"|'''Statistics without reference ''' | ||
+ | | align="center" style="background:#f0f0f0;"|'''All Data''' | ||
+ | | align="center" style="background:#f0f0f0;"|'''4 SMRT cells : 1st Set''' | ||
+ | | align="center" style="background:#f0f0f0;"|'''4 SMRT cells : 2nd Set''' | ||
+ | | align="center" style="background:#f0f0f0;"|'''4 SMRT cells : 3rd Set''' | ||
+ | | align="center" style="background:#f0f0f0;"|'''6 SMRT cells : 1st Set''' | ||
+ | | align="center" style="background:#f0f0f0;"|'''6 SMRT cells : 2nd Set''' | ||
+ | | align="center" style="background:#f0f0f0;"|'''6 SMRT cells : 3rd Set''' | ||
+ | |- | ||
+ | |# contigs||[[Media:pbcr_d6_all.fa | 2]]||[[Media:pbcr_d6_rg4_1.fa | 51]]||[[Media:pbcr_d6_rg4_2.fa | 44]]||[[Media:pbcr_d6_rg4_3.fa | 76]]||[[Media:pbcr_d6_rg6_1.fa | 6]]||[[Media:pbcr_d6_rg6_2.fa | 6]]||[[Media:pbcr_d6_rg6_3.fa | 6]] | ||
+ | |- | ||
+ | |Largest contig||3 835 938||300 354||500 180||250 542||2 045 145||2 044 223||2 542 485 | ||
+ | |- | ||
+ | |Total length||4 640 874||4 437 792||4 476 210||4 297 112||4 636 889||4 635 531||4 645 642 | ||
+ | |- | ||
+ | |N50||3 835 938||105 841||117 447||69 771||1 293 614||1 522 526||2 542 485 | ||
+ | |- | ||
+ | | style="background:#f0f0f0;"| [[Media:SCA_d6.pdf | '''Misassemblies''']]|||||||||||||| | ||
+ | |- | ||
+ | |# misassemblies||8||6||7||6||7||8||9 | ||
+ | |- | ||
+ | |Misassembled contigs length ||3 835 938||678 934||950 771||435 912||3 567 800||3 623 564||3 630 045 | ||
+ | |- | ||
+ | | style="background:#f0f0f0;"| '''Mismatches'''|||||||||||||| | ||
+ | |- | ||
+ | |# mismatches per 100kbp||0.19||1.65||1.61||3.74||0.35||0.24||0.35 | ||
+ | |- | ||
+ | |# indels per 100kbp||4.81||56.36||39.36||65.58||9.72||9.72||9.84 | ||
+ | |- | ||
+ | |# N's per 100kbp ||0||0||0||0||0||0||0 | ||
+ | |- | ||
+ | | style="background:#f0f0f0;"| '''Genome Statistics'''|||||||||||||| | ||
+ | |- | ||
+ | |Genome fraction(%) ||99.938||95.61||96.483||92.288||99.828||99.762||99.86 | ||
+ | |- | ||
+ | |Duplication ratio ||1.001||1.005||1.006||1.007||1.001||1.001||1.003 | ||
+ | |- | ||
+ | |# genes ||4490 +5 part||4229 +71 part||4288 +65 part||4094 +105 part||4481 +10 part||4475 +11 part||4485 +8 part | ||
+ | |- | ||
+ | |NGA50 ||949 276||89 654||111 935||53 222||857 569||857 671||859 217 | ||
+ | |- | ||
+ | | align="left" style="background:#f0f0f0;"|''' Running Time '''||35m 5s||17m 43s||18m 14s||16m 12s||26m 17s||26m 9s||27m 2s | ||
+ | |} | ||
{| {{table}} border="1" | {| {{table}} border="1" | ||
| align="center" style="background:#f0f0f0;"|'''Statistics without reference ''' | | align="center" style="background:#f0f0f0;"|'''Statistics without reference ''' |