(→higher coverage bias)
|
(→higher coverage bias)
|
Line 559: | |||
==higher coverage bias== | ==higher coverage bias== | ||
- | The following two pictures are the coverage distribution from eight SMRT cells of DataSet 5 and DataSet 6,and the x-axis denotes the reference genome length and the y-axis represents the coverage in the each nucleotide of reference genome. These two datasets have the similar size of long reads and over 75X depth of coverage, but the dataset 6 couldn't complete genome as correctly as dataset 5. We found that there were more regions with low coverage in dataset 6 than dataset 5. | + | The following two pictures are the coverage distribution from eight SMRT cells of DataSet 5 and DataSet 6,and the x-axis denotes the reference genome length and the y-axis represents the coverage in the each nucleotide of reference genome. These two datasets have the similar size of long reads and over 75X depth of coverage, but the dataset 6 couldn't complete genome as correctly as dataset 5. We found that there were more regions with low coverage in dataset 6 than dataset 5. The more low-coverage regions may induce the more reads couldn't be self-corrected and |