Version Differences for PBcR
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(→higher coverage bias)
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(→higher coverage bias)
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| ==higher coverage bias== | ==higher coverage bias== | ||
| - | The following two pictures are the coverage distribution from eight SMRT cells of DataSet 5 and DataSet 6,and the x-axis denotes the reference genome length and the y-axis represents the coverage in the each nucleotide of reference genome. These two datasets have the similar size of long reads and over 75X depth of coverage, but the dataset 6 couldn't complete genome as correctly as dataset 5. We found that there were more regions with low coverage in dataset 6 than dataset 5. The more low-coverage regions may induce the more reads couldn't be self-corrected so that there were not enough correctly overlapped information to assemble the contigs. Nevertheless, the upgraded RS II system increased the average read length to 5 Kbp (in Dataset 9) and expectedly provided average read lengths in excess of 10 Kbp with new chemistry (P6-C4). Besides, the continuously increased throughput would overcome the coverage bias | + | The following two pictures are the coverage distribution from eight SMRT cells of DataSet 5 and DataSet 6,and the x-axis denotes the reference genome length and the y-axis represents the coverage in each nucleotide of reference genome. These two datasets have the similar size of long reads and over 75X depth of coverage, but the dataset 6 couldn't complete genome as correctly as dataset 5. We found that there were more regions with low coverage in dataset 6 than dataset 5. The more low-coverage regions may induce the more reads couldn't be self-corrected so that there were not enough correctly overlapped information to assemble the contigs. Nevertheless, the upgraded RS II system increased the average read length to 5 Kbp (in Dataset 9) and expectedly provided average read lengths in excess of 10 Kbp with new chemistry (P6-C4). Besides, the continuously increased throughput would overcome the coverage bias |