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PBcR pipeline(S) was proposed in the [http://www.ncbi.nlm.nih.gov/pubmed/24034426 ref] (''Reducing assembly complexity of microbial genomes with single-molecule sequencin'', Genome Biology 2013). |
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PBcR pipeline(S) was proposed in the [http://www.ncbi.nlm.nih.gov/pubmed/24034426 ref] (''Reducing assembly complexity of microbial genomes with single-molecule sequencin'', Genome Biology 2013). |
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=Self-Correction Assembly=
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=PBcR pipeline=
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Following we used ''E. coli'' as an example to show the steps. All the necessary programs were downloaded from [http://www.cbcb.umd.edu/software/PBcR/closure/ cbcb] (or direct download the [ftp://ftp.cbcb.umd.edu/pub/data/PBcR/closure_paper/wgs-package.tar.gz package]) and more detail information was described at [http://sourceforge.net/apps/mediawiki/wgs-assembler/index.php?title=PacBioToCA PacBioToCA] |
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Following we used ''E. coli'' as an example to show the steps. All the necessary programs were downloaded from [http://www.cbcb.umd.edu/software/PBcR/closure/ cbcb] (or direct download the [ftp://ftp.cbcb.umd.edu/pub/data/PBcR/closure_paper/wgs-package.tar.gz package]) and more detail information was described at [http://sourceforge.net/apps/mediawiki/wgs-assembler/index.php?title=PacBioToCA PacBioToCA] |