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As described in the paper [http://www.ncbi.nlm.nih.gov/pubmed/22750884 Hybrid error correction], second-generation data can be used to correct PacBio reads and then perform ''de novo'' assembly using PacBio corrected reads (PBcR).
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As described in the paper [http://www.ncbi.nlm.nih.gov/pubmed/22750884 Hybrid error correction], second-generation data can be used to correct PacBio reads and then perform ''de novo'' assembly using PacBio corrected reads (PBcR). Here, we discuss the effects of depths on (1) hybrid error correction and (2) assembly.
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= Short read depth =
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= Hybrid error correction =
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We have used three short read depths (Raw, 118X and 100X) to correct long reads. |
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We have used three short read depths (Raw, 118X and 100X) to correct long reads. |
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We have used subreads of 1-4 SMRT cells. |
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We have used subreads of 1-4 SMRT cells. |