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As described in the paper [http://www.ncbi.nlm.nih.gov/pubmed/22750884 Hybrid error correction], second-generation data can be used to correct PacBio reads and then perform ''de novo'' assembly using PacBio corrected reads (PBcR). Here, we discuss the effects of depths on (1) hybrid error correction and (2) assembly.
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As described in the paper [http://www.ncbi.nlm.nih.gov/pubmed/22750884 Hybrid error correction], second-generation data can be used to correct PacBio reads and then perform ''de novo'' assembly using PacBio corrected reads (PBcR). Here, we discuss the effects of depths on '''(1) hybrid error correction''' and '''(2) assembly'''.
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= Hybrid error correction = |
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= Hybrid error correction = |
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After read correction, PBcR can be used to ''de novo'' assembly by runCA or Mira3.
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