(→Evaluation)
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(→Evaluation)
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We have evaluated the assemblies with [http://bioinf.spbau.ru/en/quast QUAST 2.2](reference genome and genes下載). | We have evaluated the assemblies with [http://bioinf.spbau.ru/en/quast QUAST 2.2](reference genome and genes下載). | ||
- | Single SMRT cell reads corrected with raw, 100X and 118X short reads. | + | Single SMRT cell reads were corrected with raw, 100X and 118X short reads. |
{| {{table}} border="1" | {| {{table}} border="1" | ||
| align="center" style="background:#f0f0f0;"|'''Statistics without reference''' | | align="center" style="background:#f0f0f0;"|'''Statistics without reference''' | ||
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|} | |} | ||
- | Two SMRT cell reads corrected with raw, 100X, and 118X short reads. The PBcR were then filtered to 25X or directly assembled by runCA. | + | Two SMRT cell reads were corrected with raw, 100X, and 118X short reads. The PBcR were then filtered to 25X or directly assembled by runCA. |
{| {{table}} border="1" | {| {{table}} border="1" | ||
| align="center" style="background:#f0f0f0;"|'''Statistics without reference''' | | align="center" style="background:#f0f0f0;"|'''Statistics without reference''' | ||
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|- | |- | ||
| NGA50||418393||405538||235822||193833||194196||199657 | | NGA50||418393||405538||235822||193833||194196||199657 | ||
+ | |- | ||
+ | |} | ||
+ | Three SMRT cells reads were corrected with raw, 100X, and 118 short reads. The PBcR were then filtered to 25X or directly assembled by runCA. | ||
+ | {| {{table}} border=1"1" | ||
+ | | align="center" style="background:#f0f0f0;"|'''Statistics without reference''' | ||
+ | | align="center" style="background:#f0f0f0;"|'''3_raw_asm.ctg''' | ||
+ | | align="center" style="background:#f0f0f0;"|'''3_raw_25X_asm.ctg''' | ||
+ | | align="center" style="background:#f0f0f0;"|'''3_100X_asm.ctg''' | ||
+ | | align="center" style="background:#f0f0f0;"|'''3_100X_25X_asm.ctg''' | ||
+ | | align="center" style="background:#f0f0f0;"|'''3_118X_asm.ctg''' | ||
+ | | align="center" style="background:#f0f0f0;"|'''3_118X_25X_asm.ctg''' | ||
+ | |- | ||
+ | | # contigs||219||74||98||32||86||39 | ||
+ | |- | ||
+ | | Largest contig||771076||1426293||981874||822480||1091515||520962 | ||
+ | |- | ||
+ | | Total length||5873961||5171438||5051244||4730819||4906749||4668968 | ||
+ | |- | ||
+ | | N50||247798||317846||413464||600008||286035||218547 | ||
+ | |- | ||
+ | | style="background:#f0f0f0;"| Misassemblies|||||||||||| | ||
+ | |- | ||
+ | | # misassemblies||25||10||22||8||25||11 | ||
+ | |- | ||
+ | | Misassembled contigs length||1361077||1372143||2201123||1855654||1800186||1350243 | ||
+ | |- | ||
+ | | Mismatches|||||||||||| | ||
+ | |- | ||
+ | | # mismatches per 100 kbp||4.03||2.5||2.030||1.5||4.71||5.45 | ||
+ | |- | ||
+ | | # indels per 100 kbp||1.68||0.97||5.64||3.3||4.13||3.86 | ||
+ | |- | ||
+ | | # N's per 100 kbp||0.34||0.140||0.46||0.11||0.18||0.02 | ||
+ | |- | ||
+ | | style="background:#f0f0f0;"| Genome statistics|||||||||||| | ||
+ | |- | ||
+ | | Genome fraction (%)||100||100||99.733||99.197||99.69||98.93 | ||
+ | |- | ||
+ | | Duplication ratio||1.268||1.116||1.092||1.028||1.063||1.018 | ||
+ | |- | ||
+ | | # genes||4494 + 3 part||4495 + 2 part||4484 + 9 part||4460 + 19 part||4468 + 20 part||4427 + 35 part | ||
+ | |- | ||
+ | | NGA50||286997||323732||348693||599239||286035||193471 | ||
|- | |- | ||
|} | |} |