| - |
In addition to filter 25X PBcR for assembly, we used different Celera Assembler parameters as described in [http://sourceforge.net/apps/mediawiki/wgs-assembler/index.php?title=PacBioToCA ref].
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+ |
In addition to filter 25X PBcR or no filter for assembly, we used different Celera Assembler parameters as described in [http://sourceforge.net/apps/mediawiki/wgs-assembler/index.php?title=PacBioToCA ref].
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| |
runCA -p asm -d asm -s asm.spec viaMiseq.frg |
|
runCA -p asm -d asm -s asm.spec viaMiseq.frg |
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runCA unitigger=bogart merSize=14 ovlMinLen=<ovl value> utgErrorRate=0.015 utgGraphErrorRate=0.015 utgGraphErrorLimit=0 utgMergeErrorRate=0.03 utgMergeErrorLimit=0 -p asm -d asm viaMiseq.frg |
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runCA unitigger=bogart merSize=14 ovlMinLen=<ovl value> utgErrorRate=0.015 utgGraphErrorRate=0.015 utgGraphErrorLimit=0 utgMergeErrorRate=0.03 utgMergeErrorLimit=0 -p asm -d asm viaMiseq.frg |
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+ |
We therefore had eight assemblies for comparison.
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