| |
In addition to filter 25X PBcR or no filter for assembly, we used different Celera Assembler parameters as described in [http://sourceforge.net/apps/mediawiki/wgs-assembler/index.php?title=PacBioToCA ref]. |
|
In addition to filter 25X PBcR or no filter for assembly, we used different Celera Assembler parameters as described in [http://sourceforge.net/apps/mediawiki/wgs-assembler/index.php?title=PacBioToCA ref]. |
| - |
runCA -p asm -d asm -s asm.spec viaMiseq.frg
|
+ |
'''runCA1:''' runCA -p asm -d asm -s asm.spec viaMiseq.frg
|
| - |
runCA unitigger=bogart merSize=14 ovlMinLen=<ovl value> utgErrorRate=0.015 utgGraphErrorRate=0.015 utgGraphErrorLimit=0 utgMergeErrorRate=0.03 utgMergeErrorLimit=0 -p asm -d asm viaMiseq.frg
|
+ |
'''runCA2:''' runCA unitigger=bogart merSize=14 ovlMinLen=<ovl value> utgErrorRate=0.015 utgGraphErrorRate=0.015 utgGraphErrorLimit=0 utgMergeErrorRate=0.03 utgMergeErrorLimit=0 -p asm -d asm viaMiseq.frg
|
| |
We therefore had eight assemblies for comparison. |
|
We therefore had eight assemblies for comparison. |
