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Self-correction approach (SCA) was proposed in the [http://www.ncbi.nlm.nih.gov/pubmed/24034426 ref] (''Reducing assembly complexity of microbial genomes with single-molecule sequencin'', Genome Biology 2013). |
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Self-correction approach (SCA) was proposed in the [http://www.ncbi.nlm.nih.gov/pubmed/24034426 ref] (''Reducing assembly complexity of microbial genomes with single-molecule sequencin'', Genome Biology 2013). |
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=DataSet1=
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We used all SMRT cells and randomly selected four and six SMRT cells three times for each, and access the correctness by Quast.
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==Performance==
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{| {{table}} border="1"
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| align="center" style="background:#f0f0f0;"|'''Statistics without reference '''
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| align="center" style="background:#f0f0f0;"|'''All Data'''
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| align="center" style="background:#f0f0f0;"|'''4 SMRT cells : 1st Set'''
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| align="center" style="background:#f0f0f0;"|'''4 SMRT cells : 2nd Set'''
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| align="center" style="background:#f0f0f0;"|'''4 SMRT cells : 3rd Set'''
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| align="center" style="background:#f0f0f0;"|'''6 SMRT cells : 1st Set'''
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| align="center" style="background:#f0f0f0;"|'''6 SMRT cells : 2nd Set'''
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| align="center" style="background:#f0f0f0;"|'''6 SMRT cells : 3rd Set'''
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|-
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|# contigs||16||10||14||16||9||18||13
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|-
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|Largest contig||2 198 457||3 4848 77||1 936 831||1 948 632||2 104 087||1 169 224||1 439 551
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|-
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|Total length||4 808 733||4 706 800||4 705 398||4 745 036||4 741 512||4 814 718||4 749 785
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|-
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|N50||1 005 770 ||3 484 877||966 809||1 434 284||1 655 500||676 526||1 268 010
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|-
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| style="background:#f0f0f0;"| '''Misassemblies'''||||||||||||||
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|-
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|# misassemblies||19||9||12||15||14||17||11
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|-
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|Misassembled contigs length ||2 939 040||3 530 352||2 949 761||3 653 461||3 820 624||2 387 129||3 986 402
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|-
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| style="background:#f0f0f0;"| '''Mismatches'''||||||||||||||
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|-
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|# mismatches per 100kbp||0.8||0.43||0.58||1.36||0.15||0.95||0.58
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|-
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|# indels per 100kbp||5.71||2.98||4.45||9.56||1.77||8.02||6.88
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|-
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|# N's per 100kbp ||0||0||0||0||0||0||0
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|-
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| style="background:#f0f0f0;"| '''Genome Statistics'''||||||||||||||
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|Genome fraction(%) ||100||100||99.815||99.87||100||99.995||99.979
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|-
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|Duplication ratio ||1.037||1.016||1.017||1.025||1.022||1.038||1.025
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|-
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|# genes ||4494+3 part||4494+3 part||4480+7 part||4485+9 part||4494+3 part||4493+4 part||4492+5 part
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|-
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|NGA50 ||615 234||1 205 052||572 342||875 953||844 482||633 220||1 267 242
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|-
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|'''Running Time'''||19hr 06m||13hr 34m||13hr 21m||12hr 38m||21hr 28m||22hr 56m||22hr 07m
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|}
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