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Four SMRT cells: m120228_171636 + m120228_223624 + m120228_100807 + m120228_190630 <br> |
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Four SMRT cells: m120228_171636 + m120228_223624 + m120228_100807 + m120228_190630 <br> |
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1. Generate short reads FRG file
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fastqToCA -libraryname ecoli -technology illumina -insertsize 300 30 -mates read_1.fastq,read_2.fastq > short_reads.frg
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2. Create pacbio.spec file
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PBcR -length 500 -partitions 200 -l eclo-illumina -s pacbio.spec -fastq long_reads.fastq short_reads.frg
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We have evaluated the assemblies with [http://bioinf.spbau.ru/en/quast QUAST 2.3](reference genome [ftp://ftp.ncbi.nih.gov/genomes/Bacteria/Escherichia_coli_K_12_substr__MG1655_uid57779/ NC_000913] and [[Media: Ec_gene_result.ncbi | Ec_gene_list]]). [more detail] |
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We have evaluated the assemblies with [http://bioinf.spbau.ru/en/quast QUAST 2.3](reference genome [ftp://ftp.ncbi.nih.gov/genomes/Bacteria/Escherichia_coli_K_12_substr__MG1655_uid57779/ NC_000913] and [[Media: Ec_gene_result.ncbi | Ec_gene_list]]). [more detail] |