Dataset1

Revision as of 21 August 2013 02:25 by admin (Comments | Contribs) | (PacBio corrected reads (PBcR))
Contents

Data

Short reads

The paired-end illumina read data of E. coli were downloaded from Illumina (|Illumina) with a median insert size of 214 bp. More than 28.4 M reads.

Long reads

1 SMRT Cell of 10 kbp continuous long reads (CLR) for Escherichia coli K12 MG1655 were downloaded from this link.


Assemblies with short reads only

Abyss, Edena, SPAdes, SOAPdenovo2, Velvet, CISA (Note at: 20130807_MG1655_s1s2_with_verious_Assemblers) MaSuRCA (note at: MaSuRCA assembler)

PacBio corrected reads (PBcR)

pacBioToCA -length 1000 -partitions 200 -l PacBio_Illumia -s pacbio.spec -fastq PacBio_10kb_CLR.fastq Ecoli_MG1655_PE.frg 1>PacBio_Illumia_Ecoli.out 2>error.out

Assemblies with hybrid methods

Assemble corrected long reads

runCA, MIRA3


Hybrid assemble from pre-assembled contigs and long reads

AHA, PBJelly, Cerulean, Patch