Dataset1

Eight SMRT cells of Ecoli MG1655 were downloaded from hgap.

m121023_202553_42178_c100389662550000001523034410251200_s1_p0.bas.h5 m121023_224605_42178_c100389662550000001523034410251201_s1_p0.bas.h5 m121024_010654_42178_c100389662550000001523034410251202_s1_p0.bas.h5 m121024_032737_42178_c100389662550000001523034410251203_s1_p0.bas.h5 m121024_074656_42178_c100389662550000001523034410251204_s1_p0.bas.h5 m121024_100442_42178_c100389662550000001523034410251205_s1_p0.bas.h5 m121024_122509_42178_c100389662550000001523034410251206_s1_p0.bas.h5 m121024_144608_42178_c100389662550000001523034410251207_s1_p0.bas.h5

We have run smrtpipe.py (SMRT analysis) with the following params.xml to get filtered subreads of continuous long reads (CLR).

 <param name="minLength">
   <value>50</value>
 </param>
 <param name="readScore">
   <value>0.75</value>
 </param>
 <param name="minSubReadLength">
   <value>50</value>

4個SMRT cells for Mruber, 7 個SMRT cells for Phep

17 SMRT Cells for E. coli MG1655 were downloaded (details in Data and Read Depths) and filtered with smrtpipe.