Pacbio Data

Revision as of 3 October 2013 20:23 by admin (Comments | Contribs) | (Dataset3)
Contents

Dataset1

The eight SMRT cells of Ecoli MG1655 were downloaded from HGAP.

m121023_202553_42178_c100389662550000001523034410251200_s1_p0.bas.h5
m121023_224605_42178_c100389662550000001523034410251201_s1_p0.bas.h5
m121024_010654_42178_c100389662550000001523034410251202_s1_p0.bas.h5
m121024_032737_42178_c100389662550000001523034410251203_s1_p0.bas.h5
m121024_074656_42178_c100389662550000001523034410251204_s1_p0.bas.h5
m121024_100442_42178_c100389662550000001523034410251205_s1_p0.bas.h5
m121024_122509_42178_c100389662550000001523034410251206_s1_p0.bas.h5
m121024_144608_42178_c100389662550000001523034410251207_s1_p0.bas.h5

We have run smrtpipe.py (SMRT analysis) with the following params.xml to get filtered subreads of continuous long reads (CLR).

 <param name="minLength">
   <value>50</value>
 </param>
 <param name="readScore">
   <value>0.75</value>
 </param>
 <param name="minSubReadLength">
   <value>50</value>

Dataset2

Th four SMRT cells of Mruber were downloaded from HGAP.

m120803_022519_42141_c100388772550000001523034210251234_s1_p0.bas.h5
m120803_041200_42141_c100388772550000001523034210251235_s1_p0.bas.h5
m120803_055858_42141_c100388772550000001523034210251236_s1_p0.bas.h5
m120803_074648_42141_c100388772550000001523034210251237_s1_p0.bas.h5

Dataset3

The seven SMRT cells of Phep were downloaded from HGAP.

m120802_210418_42142_c100388622550000001523034210251211_s1_p0.bas.h5
m120802_225401_42142_c100388622550000001523034210251212_s1_p0.bas.h5
m120803_004251_42142_c100388622550000001523034210251213_s1_p0.bas.h5
m120803_023226_42142_c100388622550000001523034210251214_s1_p0.bas.h5
m120803_041958_42142_c100388622550000001523034210251215_s1_p0.bas.h5
m120803_060809_42142_c100388622550000001523034210251216_s1_p0.bas.h5
m120803_075818_42142_c100388622550000001523034210251217_s1_p0.bas.h5

Dataset4

The 17 SMRT cells of Ecoli MG 1655 were downloaded from NCBI SRA, as described in Data 17 SMRT Cells for E. coli MG1655 were downloaded (details in Data and Read Depths) and filtered with smrtpipe.