As described in the paper Hybrid error correction, second-generation data can be used to correct PacBio reads and then perform de novo assembly using PacBio corrected reads (PBcR).

Short read depth

We have used three short read depths (Raw, 118X and 100X) to correct long reads.

Long read depth

We have used subreads of 1-4 SMRT cells.

PBcR depth