(→Short reads)
|
(→Long reads)
|
Line 20: | |||
We therefore obtained 1,839,935 paired-end reads (~118X, tMiSeq_PE.frg) with high quality for further analysis. | We therefore obtained 1,839,935 paired-end reads (~118X, tMiSeq_PE.frg) with high quality for further analysis. | ||
== Long reads == | == Long reads == | ||
- | 1 SMRT Cell of 10 kbp continuous long reads (CLR) for Escherichia coli K12 MG1655 were downloaded from [https://github.com/PacificBiosciences/DevNet/wiki/E%20coli%20K12%20MG1655%20Hybrid%20Assembly this link]. | + | 17 SMRT Cells for E. coli MG1655 were downloaded. |
- | The file of PacBio_10kb_CLR.fastq contains ~21X of E. coli CLR reads from a 10kb library that was filtered using standard PacBio filtering thresholds (minimum RQ=0.75, RL=50bp) ([http://files.pacb.com/datasets/secondary-analysis/e-coli-k12-de-novo/1.3.0/README.txt ref]). | ||
= Assemblies with short reads only = | = Assemblies with short reads only = | ||
Abyss, Edena, SPAdes, SOAPdenovo2, Velvet, CISA (Note at: 20130807_MG1655_s1s2_with_verious_Assemblers) | Abyss, Edena, SPAdes, SOAPdenovo2, Velvet, CISA (Note at: 20130807_MG1655_s1s2_with_verious_Assemblers) |