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In this article, we provide a comprehensive review of the above-motioned methods, and collect datasets for comparative assessment of the non-hybrid approaches—hierarchical genome-assembly process (HGAP) and self-correction approach (SCA). In addition to offering explicit and useful recommendations to practitioners, the review guides to design a project in finishing microbial genome assembly. Following a special recipe proposed by ALLPATHS-LG, to supply it with the three prepared libraries—fragment, jump and long reads, ALLPATHS-LG is able to complete microbial genomes as the sequencing coverage is controlled at 100X. Although the hybrid approach could improve the continuity over the assembly produced by the next-generation sequencing reads along, we did not successfully assemble a complete genome. The both non-hybrid approaches—HGAP and SCA—are able to produce complete genomes as long as the third generation sequencing reads are adequately long and sufficient.
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In this article, we provide a comprehensive review of the above-motioned methods, and collect datasets for comparative assessment of the non-hybrid approaches—hierarchical genome-assembly process (HGAP) and self-correction approach (SCA). <u>In addition to offering explicit and useful recommendations to practitioners, the review guides to design a project in finishing microbial genome assembly.</u> Following a special recipe proposed by ALLPATHS-LG, to supply it with the three prepared libraries—fragment, jump and long reads, ALLPATHS-LG is able to complete microbial genomes as the sequencing coverage is controlled at 100X. Although the hybrid approach could improve the continuity over the assembly produced by the next-generation sequencing reads along, we did not successfully assemble a complete genome. The both non-hybrid approaches—HGAP and SCA—are able to produce complete genomes as long as the third generation sequencing reads are adequately long and sufficient.
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