| |
|
+ |
= Completing microbial genome assemblies: strategy and performance comparisons =
|
| |
Determining the genomic sequences of microorganisms is the basis and prerequisite for understanding their biology and functional characterization. While the advent of low-cost, extremely high-throughput second-generation sequencing technologies and the parallel development of assembly algorithms have generated rapid and cost-effective genome assemblies, the assemblies are often unfinished, fragmented draft genomes as a result of the short read lengths and long repeats present in multiple copies. Several methods, such as''' ALLPATH-LG, hybrid and non-hybrid approaches''', have been proposed to utilize the third-generation sequencing long reads that can span many thousands of bases for complete microbial genome assemblies. However, there appears an insufficiency on standardized procedure for strategy comparison and evaluation on their assemblies.</font> |
|
Determining the genomic sequences of microorganisms is the basis and prerequisite for understanding their biology and functional characterization. While the advent of low-cost, extremely high-throughput second-generation sequencing technologies and the parallel development of assembly algorithms have generated rapid and cost-effective genome assemblies, the assemblies are often unfinished, fragmented draft genomes as a result of the short read lengths and long repeats present in multiple copies. Several methods, such as''' ALLPATH-LG, hybrid and non-hybrid approaches''', have been proposed to utilize the third-generation sequencing long reads that can span many thousands of bases for complete microbial genome assemblies. However, there appears an insufficiency on standardized procedure for strategy comparison and evaluation on their assemblies.</font> |
