(→Datasets employed in this study)
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(→Datasets employed in this study)
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We have conducted a <span style="color:blue;">hybrid approach</span> proposed by Koren ''et al.''([http://www.ncbi.nlm.nih.gov/pubmed/22750884 ref]) to correct long reads (<span style="color:blue;">D5</span>) with short reads (<span style="color:blue;">D4</span>) (by [http://sourceforge.net/apps/mediawiki/wgs-assembler/index.php?title=PacBioToCA PacBioToCA]), then to ''de novo'' assemble the corrected long reads (by [http://sourceforge.net/apps/mediawiki/wgs-assembler/index.php?title=runCA runCA]) for ''E. coli'' genome reconstruction. | We have conducted a <span style="color:blue;">hybrid approach</span> proposed by Koren ''et al.''([http://www.ncbi.nlm.nih.gov/pubmed/22750884 ref]) to correct long reads (<span style="color:blue;">D5</span>) with short reads (<span style="color:blue;">D4</span>) (by [http://sourceforge.net/apps/mediawiki/wgs-assembler/index.php?title=PacBioToCA PacBioToCA]), then to ''de novo'' assemble the corrected long reads (by [http://sourceforge.net/apps/mediawiki/wgs-assembler/index.php?title=runCA runCA]) for ''E. coli'' genome reconstruction. | ||
- | We have conducted the both non-hybrid approaches: <span style="background-color:#e76700;">hierarchical genome-assembly process (HGAP)</span> and <span style="color:#FF0000;background-color:#ffffcc;">self-correction approach (SCA)</span> to de novo assemble the PacBio long reads. | + | We have conducted the both non-hybrid approaches: <span style="background-color:#D4F2CE;">hierarchical genome-assembly process (HGAP)</span> and <span style="color:#FF0000;background-color:#ffffcc;">self-correction approach (SCA)</span> to de novo assemble the PacBio long reads. |
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