Data

Short reads

Paired reads are available at Illumina Miseq (Mate1, Mate2)

Read length: 151bp

Read amount: 5,729,470 X2

Insert size ~ 300bp

We converted the required format using Picard tools (ref).

java -jar SamToFastq.jar INPUT=Ecoli_MG1655_s_6_1_bfast.bam FASTQ=Ecoli_MG1655_s1.fastq
java -jar SamToFastq.jar INPUT=Ecoli_MG1655_s_6_2_bfast.bam FASTQ=Ecoli_MG1655_s2.fastq

We combined MiSeq_Ecoli_MG1655_110721_PF_R1.fastq and MiSeq_Ecoli_MG1655_110721_PF_R2.fastq to MiSeq_PE.fastq.

java convertFastqToFastaAndQual MiSeq_PE.fastq MiSeq_PE.fna MiSeq_PE.qual
convert-fasta-to-v2.pl -mean 214 -stddev 21 -m Mate_info -l Illumia_Ecoli -s Ecoli_MG1655_PE.fna -q Ecoli_MG1655_PE.qual > Ecoli_MG1655_PE.frg

Long reads

1 SMRT Cell of 10 kbp continuous long reads (CLR) for Escherichia coli K12 MG1655 were downloaded from this link.

The file of PacBio_10kb_CLR.fastq contains ~21X of E. coli CLR reads from a 10kb library that was filtered using standard PacBio filtering thresholds (minimum RQ=0.75, RL=50bp) (ref).

Assemblies with short reads only

Abyss, Edena, SPAdes, SOAPdenovo2, Velvet, CISA (Note at: 20130807_MG1655_s1s2_with_verious_Assemblers) MaSuRCA (note at: MaSuRCA assembler)

PacBio corrected reads (PBcR)

To correct the PacBio CLR with raw short reads:

pacBioToCA -length 1000 -partitions 200 -l PacBio_Illumia -s pacbio.spec -fastq PacBio_10kb_CLR.fastq Ecoli_MG1655_PE.frg 1>PacBio_Illumia_Ecoli.out 2>error.out

To correct the PacBio CLR with 100X high-quality reads (p<0.05, length of paired-end read>=100bp)

pacBioToCA -length 500 -partitions 200 -l PacBio_Illumia -s pacbio.spec -fastq PacBio_10kb_CLR.fastq 100X_Ecoli_PE.frg

Assemblies with hybrid methods

Assemble corrected long reads

runCA (20130807_MG1655_s1s2_with_verious_Assemblers for 100X, 20120628_20120628_PacBio_With_CA(Celera Assembler)_Wgs), MIRA3

Hybrid assemble from pre-assembled contigs and long reads

AHA, PBJelly, Cerulean, Patch